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casanova Experiment

Page 4: Data

Now you are going to get the chance to see live action videos of zebrafish cas mutants and determine for yourself if the presented hypothesis is supported or refuted by the data. It is recomended that you test your hypothesis by observing the bpm (beats per minute) of the cas mutant and WT (wild type) fish. To do this it would be handy to have some sort of time keeping device (a regular watch or cell phone would do just fine). The WT is included so that a comparison can be made between the casanova mutant heart beat and the wild type.

These videos were generated using zebrafish that had the actin promoter driving Green Fluorescent Protein (GFP) production. The larva were mounted in methylcellulose in order to keep their movement down to a minimum.

 

Movie of a beating heart in a WT embryo (~1.5 days old)

WT Movie #1

Movies of beating hearts in casanova mutants (~1.5 days old)

 

cas movie #1

cas movie #2

cas movie #3

cas movies #4

 

Newer Movies of beating hearts in casanova mutants (bright field) (~1.5 days old)

Legends for new movies

WT movie #2

WT movie #3

cas movie #5

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