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Outreach to 5th grade science class

This lesson plan was developed for a 45 minute visit to a 5th grade science classrom. Students move through 4 stations, each with a different theme. We presented this two times. The themes for the first iteration were Phenotype, Genotype, Inheritance, and Fluorescence. Rhw themes for the second iteration were Phenotype, Inheritance, Fluorescence, and Metamorphosis. The Phenotype, Genotype, Inheritance, and Fluorescence stations each had a different set of zebrafish for the students to use in learning each concept. The metamorphosis station used Menduca sexta moths/tobacco horned worm caterpillars. The zebrafish stations primarily used GloFish, which are available at most pet stores, and which can be bred for educational purposes.

Authors: Amelia Anderson, Alisa Brakic, Jayce Brown,
Rebecca Goeman, Mitch Johnson, Alison Kingsbury, Jennifer Liang, Morgan Prochaska

General materials:
Timeline and plan for first 5th grade visit (Marshall School)
Timeline and plan for second 5th grade visit (Congdon Park Elementary School)

Assessment for first 5th grade visit
Assessment for second 5th grade visit

Titles for 5th grade stations-ppt
Titles for 5th grade stations-pdf

Phenotype station

tank of fish with many different colors and pigment patternstank of fish with many colors and pigment patterns

Almost every pet store sells GloFish as well as normal, wildtype zebrafish
GloFish website: http://www.glofish.com

Plan for Inheritance Station (docx)
Plan for Inheritance Station (pdf)

Genotype station

student notebook page with work on recessive and dominant alleles

Plan for genotype station (docx)
Plan for genotype station (pdf)

Fillable punnet square (print out an laminate-use dry erase markers to fill in squares)

Fluorescence/GloFish station

Student looking at GloFish with blacklight flashlight

Plan for Fluorescence Station (docx)
Plan for Fluorescence Station (pdf)

Print out and laminate (all printout include references for information and images)
Examples of fluorescent animals scientists have made (ppt)
Examples of fluorescent animals scientists have made (pdf)
How GloFish are made (ppt)
How GloFish are made (pdf)
Brainbow fish (ppt)
Brainbow fish (pdf)

Sources for buying GloFish and black light flashlights:
Almost every pet store sells GloFish as well as normal, wildtype zebrafish
GloFish website: http://www.glofish.com
Blacklight flashlights to make fish glow: LEDwholesalers 395 nM 51 UV Ultraviolet LED flashlight Blacklight 3 AA, 7202UV395.
There are also many other brands of blacklight flashlights that will work.
We use a piece of black fleece for the kids to duck under to see the fish glow.

Inheritance Station

closeup of zebrafishset up of inheritance station with matching tanks of parental and progeny fish

Plan for Inheritance Station (docx)
Plan for Inheritance Station (pdf)

Note: GloFish can be bred for educational purposes. Forr complete information go to:
http://www.glofish.com/our-company/license-notice/

Hypothesis/Metamorphosis Moth Station

pictures of caterpillars at different developmental stagesmorgan holding a moth

Plan for Hypothesis Station (docx)
Plan for Hypothesis Station (pdf)

Print out and laminate:
pdf with different stages of Menduca sexta/tobacco horned worm development
These are original pictures taken by Jennifer Liang

Source for Manduca sexta caterpillars:
Carolina Biological
http://www.carolina.com/catalog/
Search for "Manduca sexta". You can now buy eggs and grow them all the way to moths in your classroom.
However, they are not available to people in some states, so you may have to find an alternative organism for this station